Rat Anti-CD5 Recombinant Antibody (clone 53-7.3)

Name: Rat Anti-CD5 Recombinant Antibody (clone 53-7.3)
SKU: NEUT-392CQ
Rating: 5 (1 reviews)

CAT#: NEUT-392CQ

This antibody is a rat monoclonal antibody that binds specifically to mouse CD5, and it can neutralize the bioactivity of mouse CD5.

Gene Expression Tested Data

Normal Tissue Figure 1 Testis

Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_6.jpg

Normal Tissue Figure 2 Lymph node

Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_7_8.jpg

Normal Tissue Figure 3 Tonsil

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43416/101306_A_5_8.jpg

RNA Expression Figure 4 RNA cell line category: Group enriched (JURKAT, MOLT-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000110448-CD5

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Figure 1 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in WB

Western blot analysis of NEUT-392CQ-Fab was performed by loading recombinant mouse CD5 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with purified antibody and HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.

Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)

ELISA

Figure 2 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in ELISA

ELISA analysis of NEUT-392CQ-Fab was performed by coating with recombinant mouse CD5 protein. Then blocked with BSA and incubated with NEUT-392CQ-Fab. The HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

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Specifications

Immunogen
Spleen cells or thymocyte membranes

Host Species
Rat

Type
Rat IgG2a, κ

Specificity
Mouse CD5

Species Reactivity
Mouse

Clone
53-7.3

Applications
FC, IHC-Fr, IP, ELISA, Block

Conjugate
Unconjugated

Product Property

Purity
>95% as determined by analysis by SDS-PAGE

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Cd5; CD5 antigen; Ly-1; Ly-A; Ly-12; Lyt-1

Gene ID
12507

UniProt ID
P13379

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(Creative Biolabs Cat# NEUT-392CQ, RRID: AB_3111570)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - NEUT-392CQ.pdf

MSDS

MSDS - NEUT-392CQ.pdf

COA

Certificate of Analysis Lookup

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