Rat Anti-CD5 Recombinant Antibody (clone 53-7.3) (CAT#: NEUT-392CQ)
This antibody is a rat monoclonal antibody that binds specifically to mouse CD5, and it can neutralize the bioactivity of mouse CD5.
Specific Inquiry
WB
Figure 1 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in WB
Western blot analysis of NEUT-392CQ-Fab was performed by loading recombinant mouse CD5 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with purified antibody and HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.1 μg)
Lane 2: Reduced antigen (0.3 μg)
Lane 3: Reduced antigen (0.6 μg)
ELISA
Figure 2 Anti-CD5 Antibody (53-7.3) Fab Fragment (NEUT-392CQ-Fab) in ELISA
ELISA analysis of NEUT-392CQ-Fab was performed by coating with recombinant mouse CD5 protein. Then blocked with BSA and incubated with NEUT-392CQ-Fab. The HRP conjugated Goat Anti-Rat IgG (H+L) as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Spleen cells or thymocyte membranes
- Host Species
- Rat
- Type
- Rat IgG2a, κ
- Specificity
- Mouse CD5
- Species Reactivity
- Mouse
- Clone
- 53-7.3
- Applications
- FC, IHC-Fr, IP, ELISA, Block
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| TAB-884 | Mouse Anti-CD5 Recombinant Antibody (clone Zolimomab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | Mouse IgG1 |
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| TAB-308LC-F(E) | Anti-Human CD5 Recombinant Antibody Fab Fragment (5D7) | FC, SPR |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# NEUT-392CQ, RRID: AB_3111570)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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